Article

CRISPR-Cas9 Targeting of G-Quadruplex DNA in ADH1 promoter Highlights its role in Transcriptome and Metabolome Regulation

2025-03-14

Abstract excerpt

<h4>SUMMARY</h4> G-quadruplex (G4) structures are critical regulators of gene expression, yet the role of an individual G4 within its native chromatin remains underexplored. Here, we used CRISPR-Cas9 to introduce guanine-to-thymine mutations at a G4-forming motif within the adh1 + promoter in yeast, creating two mutant strains: one with G4-only mutations and another with both G4 and TATA-box mutations. Chromati...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f59b8033-bb03-5fd5-929e-59ee0d998b49
DOI: 10.1101/2025.03.14.643214

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR-Cas9 Targeting of G-Quadruplex DNA in ADH1 promoter Highlights its role in Transcriptome and Metabolome RegulationDOI 10.1101/2025.03.14.643214Read

Metabolic disease variants rewire gene regulation through disruption of DNA G-quadruplex structures.Semantic proximity Read

Guide for phenotype-specific profiling of DNA G-quadruplex-regulated genes.Semantic proximity Read

SNPs associated with Metabolic Disorders Disrupt Structural Properties of DNA G-Quadruplexes in Regulatory Regions relevant during Tumor DevelopmentSemantic proximity Read

The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast <i>S. cerevisiae</i>Semantic proximity Read

G-Quadruplex-Protein Interactome at Human Gene PromotersSemantic proximity Read

G-quadruplex structural variations in human genome associated with single-nucleotide variations and their impact on gene activity.Semantic proximity Read

Detect Genomic G-Quadruplexes in Living Animal Cells with a Tiny Artificial Protein ProbeSemantic proximity Read

Chromatin regulator perturbation causes heme pathway imbalance in yeast and human cellsSemantic proximity Read

Genome-wide mapping of DNA G-quadruplexes in <i>Trypanosoma brucei</i> chromatin reveals enrichment in coding regionsSemantic proximity Read

Genetic variations in G-Quadruplex forming sequences affect the transcription of human disease-related genesSemantic proximity Read

Effects of single and double mutations on the MYC promoter G-quadruplex using a custom G4 DNA microarray: conformational landscape and nearby guanine compensation.Semantic proximity Read

Analysis of nucleotide variations in human g-quadruplex forming regions associated with disease statesSemantic proximity Read