Article

Cell type-specific translational regulation by human DUS enzymes

2023-11-03

Abstract excerpt

<h4>ABSTRACT</h4> Dihydrouridine is an abundant and conserved modified nucleoside present on tRNA, but characterization and functional studies of modification sites and associated DUS writer enzymes in mammals is lacking. Here we use a chemical probing strategy, RNABPP-PS, to identify 5-chlorouridine as an activity-based probe for human DUS enzymes. We map D modifications using RNA-protein crosslinking and chemic...

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Literature Corpus work: f17a4f74-d799-5c0a-8c0f-dc3e11adcaf5
DOI: 10.1101/2023.11.03.565399

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