Article

Quantitative profiling of pseudouridylation landscape in the human transcriptome

2022-10-25

Abstract excerpt

Pseudouridine (Ψ) is an abundant post-transcriptional RNA modification in ncRNA and mRNA. However, transcriptome-wide measurement of individual Ψ sites remains unaddressed. Here, we develop “PRAISE”, via selective chemical labeling of Ψ by bisulfite to induce nucleotide deletion signature during reverse transcription, to realize quantitative assessment of the Ψ landscape in the human transcriptome. Unlike traditio...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 49333f6c-b0b7-54bb-962f-c234c431180e
DOI: 10.1101/2022.10.25.513650

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Quantitative profiling of pseudouridylation landscape in the human transcriptomeDOI 10.1101/2022.10.25.513650Read

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptomeSemantic proximity Read

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolutionSemantic proximity Read

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptomeSemantic proximity Read

Exploring pseudouridylation: dysregulation in disease and therapeutic potentialSemantic proximity Read

Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect splicingSemantic proximity Read

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct and long-read sequencingSemantic proximity Read

Mechanistic insight into the pseudouridylation of RNASemantic proximity Read

Recent developments, opportunities, and challenges in the study of mRNA pseudouridylationSemantic proximity Read

A Radiolabeling-Free, qPCR-Based Method for Locus-Specific Pseudouridine Detection.Semantic proximity Read

Cytoplasmic localization of PUS7 facilitates a pseudouridine-dependent enhancement of cellular stress toleranceSemantic proximity Read

Enzyme-mediated alkynylation enables transcriptome-wide identification of pseudouridine modificationsSemantic proximity Read

Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based techniqueSemantic proximity Read