Article

Promoted Read-through and Mutation Against Pseudouridine-CMC by an Evolved Reverse Transcriptase

2024-07-03

Abstract excerpt

<h4>ABSTRACT</h4> Pseudouridine (Ψ) is an abundant RNA chemical modification that can play critical roles in the biological functions of RNA, and RNA-therapeutic applications. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. The N-cyclohexyl N’-(2-morpholinoethyl)carbodiimide (CMC) can selectively label Ψ in RNA by forming the CMC...

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Literature Corpus work: 5c2ef8ae-8249-52b1-848a-35f8417da5de
DOI: 10.1101/2024.07.03.601893

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