Article

Synthesizing unmodified, supercoiled circular DNA molecules <i>in vitro</i>

2025-01-25

Abstract excerpt

Supercoiled (Sc) circular DNA, such as plasmids, has shown therapeutic potential since the 1990s, but is limited by bacterial modifications, unnecessary DNA sequences, and contaminations that may trigger harmful responses. To overcome these challenges, we have developed two novel scalable biochemical methods to synthesize unmodified Sc circular DNA. Linear DNA with two loxP sites in the same orientation is generat...

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Identifiers and source

Literature Corpus work: f112b45d-1afe-50ac-b1ef-269112f8769b
DOI: 10.1101/2025.01.24.634800

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