Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors

7 Sept 2022

Abstract excerpt

While genome editing has been revolutionized by the advent of CRISPR-based nucleases, difficulties in achieving efficient, nuclease-mediated, homology-directed repair (HDR) still limit many applications. Commonly used DNA donors such as plasmids suffer from low HDR efficiencies in many cell types, as well as integration at unintended sites. In contrast, single-stranded DNA (ssDNA) donors can produce efficient HDR...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Mosquito-borne diseases and control
Share to Plant Virus Research Studies

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 36070530
DOI: 10.1089/crispr.2022.0058

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Efficient Homology-Directed Repair with Circular Single-Stranded DNA DonorsPMID 36070530Read ↗

Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineeringSemantic proximity Read ↗

Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingSemantic proximity Read ↗

Design and specificity of long ssDNA donors for CRISPR-based knock-inSemantic proximity Read ↗

ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid cellsSemantic proximity Read ↗

Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cellsSemantic proximity Read ↗

High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.Semantic proximity Read ↗

Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of genetic payloads for rapid cell engineeringSemantic proximity Read ↗

Single-stranded HDR templates with truncated Cas12a binding sequences improve knock-in efficiencies in primary human T cellsSemantic proximity Read ↗

Phage DNA polymerase prevents deleterious on-target DNA damage and enhances precise CRISPR/Cas9 editingSemantic proximity Read ↗

Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cellsSemantic proximity Read ↗

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaksSemantic proximity Read ↗

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome IntegrationSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.