Article

Rapid and scalable <i>in vitro</i> production of single-stranded DNA

2019-02-22

Abstract excerpt

We present a rapid, scalable, user-friendly method for in vitro production of high-purity single-stranded DNA (ssDNA) ranging from 89–3315 nucleotides in length. PCR with a forward primer bearing a methanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the modified strand under denaturing conditions. We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homol...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: b769d7d8-87f9-5c30-af35-519176ef3707
DOI: 10.1101/558429

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Rapid and scalable <i>in vitro</i> production of single-stranded DNADOI 10.1101/558429Read

Short single-stranded DNA oligonucleotides enable intracellular transcription of functional RNAs in mammalian cellsSemantic proximity Read

Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineeringSemantic proximity Read

A Robust and Scalable Workflow for the Production of Circular Single-Stranded DNA for Genome Engineering ApplicationsSemantic proximity Read

High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails.Semantic proximity Read

<i>In cellulo</i> DNA assembly for targeted genomic integration and rearrangement in human cellsSemantic proximity Read

Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaksSemantic proximity Read

A spring-loaded and leakage-tolerant synthetic gene switch for in-vitro detection of DNA and RNASemantic proximity Read

Dynamic aqueous multiphase reaction system for simple, sensitive and quantitative one-pot CRISPR-Cas12a based molecular diagnosisSemantic proximity Read

CRISPR-Cas9 mediated nuclear transport and genomic integration of nanostructured genes in human primary cellsSemantic proximity Read

Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNASemantic proximity Read

Ultra-large targeted DNA integrations in primary human cellsSemantic proximity Read

Precision DNA Impurity Reduction Approaches for Ultra-Pure rAAV ManufacturingSemantic proximity Read