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Article

Ultra-large targeted DNA integrations in primary human cells

2026-04-09

Abstract excerpt

<h4>ABSTRACT</h4> Genetic engineering experiments and therapies are constrained by the size of DNA integrations into human cell’s genomes. Existing AAV, lentiviral, and non-viral methods rapidly decrease in integration efficiency beyond ∼5kb of sequence. Through systematic evaluation of non-viral DNA template formats, we identified circular ssDNA and dsDNA as capable of mediating >5kb integrations. Large circula...

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Literature Corpus work
aa5c5627-20a5-58f3-9e32-0e82f2429154
DOI
10.64898/2026.04.09.717505
Open publication

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Ultra-large targeted DNA integrations in primary human cellsDOI 10.64898/2026.04.09.717505
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