Article
A rapid method for site-specific mutagenesis and directional subcloning by using the polymerase chain reaction to generate recombinant circles.
BioTechniques - 1 Feb 1990
Jones D H, Howard B H
Abstract excerpt
Site-specific mutagenesis and directional subcloning were accomplished by using the polymerase chain reaction to generate products that can recombine to form circular DNA. This DNA was transfected into E. coli without phosphorylation of primers, restriction enzyme digestion or ligation. Specifically, the polymerase chain reaction was used to generate products that when combined, denatured and reannealed, form...
Topics
- Base Sequence
- Cloning, Molecular
- DNA, Circular
- DNA, Recombinant
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Nucleic Acid Amplification Techniques
- Polymerase Chain Reaction
- Transfection