Article

Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering

2022-12-02

Abstract excerpt

The toolbox for genome editing in basic research and therapeutic applications is rapidly expanding. While efficient targeted gene ablation using nuclease editors has been demonstrated from bench to bedside, precise transgene integration remains a technical challenge. AAV6 has been a prevalent donor carrier for homology-directed repair (HDR) mediated genome engineering but has reported safety issues, manufacturing...

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Literature Corpus work: 803016c9-eda0-5bdb-8951-0c7aca16bf89
DOI: 10.1101/2022.12.01.518578

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