Article

Circularization of <i>rv0678</i> for genotypic bedaquiline resistance testing of <i>Mycobacterium tuberculosis</i>

2022-10-06

Abstract excerpt

Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlined methods for generating PCR-targeted circular DNA from a 700bp amplicon of rv0678 , the high GC content (65%) gene implicated in Mycobacterium tuberculosis bedaqui...

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Literature Corpus work: 89913a6b-26c6-5eeb-a9ad-d75514cdd5f0
DOI: 10.1101/2022.10.05.511060

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