Article

Neutrophils cultured<i>ex vivo</i>from CD34<sup>+</sup>stem cells are immature and genetically tractable

2023-07-13

Abstract excerpt

Neutrophils are essential antimicrobial effector cells with short lifespans. During infection or sterile inflammation, accelerated production and release of immature neutrophils from the bone marrow serves to boost circulating neutrophil counts. To facilitate the study of neutrophil development and function, we optimised a method for ex vivo production of human neutrophils from CD34 + haematopoietic progenitors. W...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: efef76b8-829e-51b3-bf55-4e97f774df1b
DOI: 10.1101/2023.07.12.23292345

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Neutrophils cultured<i>ex vivo</i>from CD34<sup>+</sup>stem cells are immature and genetically tractableDOI 10.1101/2023.07.12.23292345Read

Neutrophils cultured ex vivo from CD34+ stem cells are immature and genetically tractable.Semantic proximity Read

Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsSemantic proximity Read

Epigenomic and functional dynamics of human bone marrow myeloid differentiation to mature blood neutrophilsSemantic proximity Read

Cas9 <sup>+</sup> conditionally immortalized neutrophil progenitors as a tool for genome wide CRISPR screening for neutrophil differentiation and functionSemantic proximity Read

The in vitro production and characterization of neutrophils from embryonic stem cells.Semantic proximity Read

<i>GATA1</i> knockout in human pluripotent stem cells generates enhanced neutrophils to investigate extracellular trap formationSemantic proximity Read

<i>GATA1</i> Knockout in Human Pluripotent Stem Cells Generates Enhanced Neutrophils to Investigate Extracellular Trap FormationSemantic proximity Read

Single-cell transcriptome profiling reveals neutrophil heterogeneity and orchestrated maturation during homeostasis and bacterial infectionSemantic proximity Read

Infection-experienced HSPCs protect against infections by generating neutrophils with enhanced bactericidal activitySemantic proximity Read

GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18Semantic proximity Read

Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapySemantic proximity Read

In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.Semantic proximity Read