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Article

The in vitro production and characterization of neutrophils from embryonic stem cells.

Blood - 1 Feb 2004

Lieber Jonathan G, Webb Saiphone, Suratt Benjamin T, Young Scott K, Johnson Gary L, Keller Gordon M, Worthen G Scott

Abstract excerpt

An embryonic stem (ES) cell/OP9 coculture system for the effective production of functional neutrophils is described. A 3-step differentiation strategy was developed that uses liquid culture, enabling reliable and abundant production of neutrophils at high purity without the need of sorting for isolation of mature neutrophils. Use of the OP9 stromal cell line significantly enhances the number, percentage, and...

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PMID: 14525782
DOI: 10.1182/blood-2003-04-1030

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MIP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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