Article

Quantitative analysis shows that repair of Cas9-induced double-strand DNA breaks is slow and error-prone

2017-05-26

Abstract excerpt

<h4>Summary</h4> The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is known about the kinetics and fidelity of the double-strand break (DSB) repair process that follows a Cas9 cutting event in living cells. Here, we developed a strategy to measure the kinetics of DSB repair for single loci in human cells. Quantitative modeling of repaired DNA in time series after Cas9 activation r...

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Literature Corpus work: edea585a-53ef-5553-a858-d49a6baedb5b
DOI: 10.1101/142802

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