Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair.

Scientific reports - 30 Sept 2021

Schubert Mollie S, Thommandru Bernice, Woodley Jessica, Turk Rolf, Yan Shuqi, Kurgan Gavin, McNeill Matthew S, Rettig Garrett R

Abstract excerpt

CRISPR-Cas proteins are RNA-guided nucleases used to introduce double-stranded breaks (DSBs) at targeted genomic loci. DSBs are repaired by endogenous cellular pathways such as non-homologous end joining (NHEJ) and homology-directed repair (HDR). Providing an exogenous DNA template during repair allows for the intentional, precise incorporation of a desired mutation via the HDR pathway. However, rates of repair...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to CRISPR-Associated Protein 9

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Associated Proteins

Share to CRISPR-Cas Systems

Share to Endodeoxyribonucleases

Share to Gene Editing

Share to Recombinational DNA Repair

Share to RNA, Guide, CRISPR-Cas Systems

Source

Open in PubMed Open full text

Identifiers

PMID: 34593942
DOI: 10.1038/s41598-021-98965-y
PMCID: PMC8484621

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair.PMID 34593942Read ↗

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future InnovationsSemantic proximity Read ↗

Disruptive Technology: CRISPR/Cas-Based Tools and ApproachesSemantic proximity Read ↗

Design and specificity of long ssDNA donors for CRISPR-based knock-inSemantic proximity Read ↗

Utilization of nicking properties of CRISPR-Cas12a effector for genome editing.Semantic proximity Read ↗

Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair.Semantic proximity Read ↗

Utilization of nicking properties of CRISPR-Cas12a effector for genome editingSemantic proximity Read ↗

Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption.Semantic proximity Read ↗

Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editingSemantic proximity Read ↗

Analysis of NHEJ-Based DNA Repair after CRISPR-Mediated DNA Cleavage.Semantic proximity Read ↗

Rational Design of ssODN to Correct Mutations by Gene Editing.Semantic proximity Read ↗

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.Semantic proximity Read ↗

Altered DNA repair pathway engagement by engineered CRISPR-Cas9 nucleases.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.