Article

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks

2022-11-16

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas9, an efficient genome editing tool, has been widely used in research and holds great promise in the clinic. However, large unintended rearrangements of the genome occur frequently after CRISPR-Cas9 editing and their potential risk cannot be ignored. In this study, we detected large deletions (LDs) induced by CRISPR-Cas9 in human embryonic stem cells (hESCs) and found the microhomology...

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Literature Corpus work: 659adacc-0cf0-56d9-a8d8-cccc54bff50b
DOI: 10.1101/2022.11.16.516713

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