Article

Covalent linkage of the DNA repair template to the CRISPR/Cas9 complex enhances homology-directed repair

2017-11-11

Abstract excerpt

The CRISPR/Cas9 targeted nuclease technology allows the insertion of genetic modifications with single base-pair precision. The preference of mammalian cells to repair Cas9-induced DNA double-strand breaks via non-homologous end joining (NHEJ) rather than via homology-directed repair (HDR) however leads to relatively low rates of correctly edited loci. Here we demonstrate that covalently linking the DNA repair tem...

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Literature Corpus work: 6cf47a8f-7b5d-5bb1-92cb-8e0be58693db
DOI: 10.1101/218149

Open publication

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