Article
Altered DNA repair pathway engagement by engineered CRISPR-Cas9 nucleases.
Proceedings of the National Academy of Sciences of the United States of America - 14 Mar 2023
Chauhan Vikash P, Sharp Phillip A, Langer Robert
Abstract excerpt
CRISPR-Cas9 introduces targeted DNA breaks that engage competing DNA repair pathways, producing a spectrum of imprecise insertion/deletion mutations (indels) and precise templated mutations (precise edits). The relative frequencies of these pathways are thought to primarily depend on genomic sequence and cell state contexts, limiting control over mutational outcomes. Here, we report that engineered Cas9 nucleases...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.