Article

A simple cloning-free method to efficiently induce gene expression using CRISPR/Cas9

2018-08-27

Abstract excerpt

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiological expression levels. The rapid development of CRISPR/Cas technology presents promising opportunities to address these issues. Here we report a simple, cloning-free method to induce gene expression at endogenous locus using CRISPR/Cas9 activators. Our strategy utilises synthesized sgRN...

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Literature Corpus work: edade100-2330-5334-b092-495d31c9e9be
DOI: 10.1101/400838

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