Article

Collateral cleavage of 28s rRNA by RfxCas13d causes death of mice

2022-01-18

Abstract excerpt

<h4>Summary</h4> The CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d in the adult brain neurons caused death of mice, which was not resul...

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Identifiers and source

Literature Corpus work: 95b56577-d67c-598a-90c4-e26901726584
DOI: 10.1101/2022.01.17.476700

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