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Article

Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells.

Development (Cambridge, England) - 15 Feb 2017

Bressan Raul Bardini, Dewari Pooran Singh, Kalantzaki Maria, Gangoso Ester, Matjusaitis Mantas, Garcia-Diaz Claudia, Blin Carla, Grant Vivien, Bulstrode Harry, Gogolok Sabine, Skarnes William C, Pollard Steven M

Abstract excerpt

Mammalian neural stem cell (NSC) lines provide a tractable model for discovery across stem cell and developmental biology, regenerative medicine and neuroscience. They can be derived from foetal or adult germinal tissues and continuously propagated in vitro as adherent monolayers. NSCs are clonally expandable, genetically stable, and easily transfectable - experimental attributes compatible with targeted genetic...

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Identifiers

PMID: 28096221
DOI: 10.1242/dev.140855
PMCID: PMC5312033

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Source receipt: pubmed, retrieved 7 Sept 2026.

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