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Article

CRISPR-Cas12a-assisted PCR tagging of mammalian genes.

The Journal of cell biology - 1 Jun 2020

Fueller Julia, Herbst Konrad, Meurer Matthias, Gubicza Krisztina, Kurtulmus Bahtiyar, Knopf Julia D, Kirrmaier Daniel, Buchmuller Benjamin C, Pereira Gislene, Lemberg Marius K, Knop Michael

Abstract excerpt

Here we describe a time-efficient strategy for endogenous C-terminal gene tagging in mammalian tissue culture cells. An online platform is used to design two long gene-specific oligonucleotides for PCR with generic template cassettes to create linear dsDNA donors, termed PCR cassettes. PCR cassettes encode the tag (e.g., GFP), a Cas12a CRISPR RNA for cleavage of the target locus, and short homology arms for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32406907
DOI: 10.1083/jcb.201910210
PMCID: PMC7265327

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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