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Genetic editing of primary human dorsal root ganglion neurons using CRISPR-Cas9

1 Apr 2025

Abstract excerpt

CRISPR-Cas9 is now the leading method for genome editing and is advancing for the treatment of human disease. CRIPSR has promise in treating neurological diseases, but traditional viral-vector-delivery approaches have neurotoxicity limiting their use. Here we describe a simple method for non-viral transfection of primary human DRG (hDRG) neurons for CRISPR-Cas9 editing. We edited TRPV1 , NTSR2 , and CACNA1E using...

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PMID: 40169710
DOI: 10.1038/s41598-025-91153-2

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