Article

Generation and Validation of Versatile Inducible CRISPRi Embryonic Stem Cell and Mouse Models

2020-04-28

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation of the epigenome and controlling of gene expression. However the large transgene size of dCas9-fusion hinders its applications especially in somatic tissues. Here, we develop a robust CRISPR interference (CRISPRi) system by transgenic expression of doxyc...

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Literature Corpus work: b6d901da-f0b6-5618-a902-9986d100421c
DOI: 10.1101/2020.04.28.065854

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