Back to search

Article

RAPID: Evaluation of Cas12a Protospacer Nicking and Chimeric Reporters for PAM-independent RNA and DNA diagnostics

2025-07-14

Abstract excerpt

CRISPR-Cas nucleases have revolutionized diagnostics and biotechnology by providing programmable specificity. Here, we extend the understanding of Cas12a biology with a screen that, unexpectedly, finds that Cas12a trans cleavage activity can be modulated by nicks in the protospacer in a position-dependent manner. Wanting to explore the impact of non-conventional trans cleavage substrates, we subsequently find th...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
eb0ab124-a315-5ded-8dd8-2da7f97c4929
DOI
10.1101/2025.07.12.25331452
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
RAPID: Evaluation of Cas12a Protospacer Nicking and Chimeric Reporters for PAM-independent RNA and DNA diagnosticsDOI 10.1101/2025.07.12.25331452
Harnessing Non-Canonical crRNAs to Improve Functionality of Cas12a OrthologsSemantic proximity
Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnosticsSemantic proximity
A Novel Dual-Guide CRISPR-Cas13 Strategy Improves Specificity for Single-Nucleotide Variant DetectionSemantic proximity
A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNASemantic proximity
Modular split-trigger cooperative activation enables a programmable Cas13-12 cascadeSemantic proximity
A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical SamplesSemantic proximity
From RNA to DNA: CRISPR/LbuCas13a Demonstrates Exceptional Single-Nucleotide SpecificitySemantic proximity
Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection.Semantic proximity
PAM-free diagnostics with diverse type V CRISPR-Cas systemsSemantic proximity
PAM-free diagnostics with diverse type V CRISPR-Cas systemsSemantic proximity
A novel Dual-guide CRISPR-Cas13 strategy improves specificity for single-nucleotide variant detection.Semantic proximity
Accelerated RNA detection using tandem CRISPR nucleasesSemantic proximity
Select a neighboring publication to make it the new centre.