Article

PAM-free diagnostics with diverse type V CRISPR-Cas systems

2024-05-03

Abstract excerpt

Type V CRISPR-Cas effectors have revolutionized molecular diagnostics by facilitating the detection of nucleic acid biomarkers. However, their dependence on the presence of protospacer adjacent motif (PAM) sites on the target double-stranded DNA (dsDNA) greatly limits their flexibility as diagnostic tools. Here we present a novel method named PICNIC that solves the PAM problem for CRISPR-based diagnostics with jus...

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Literature Corpus work: fc5dec9f-2029-5d5f-aea3-e5829749f1b1
DOI: 10.1101/2024.05.02.24306194

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