Article
A novel Dual-guide CRISPR-Cas13 strategy improves specificity for single-nucleotide variant detection.
Nucleic acids research - 24 Feb 2026
Aguilar-González Araceli, Martos-Jamai Ismael, Ramos-Hernández Iris, Molina-Estévez Francisco Javier, Villao Nancy Villegas, Puig-Serra Pilar, Rodríguez-Perales Sandra, Torres Raúl, Labun Kornel, Sánchez-Martín Rosario María, Díaz-Mochón Juan José, Martín Francisco
Abstract excerpt
The emergence of CRISPR-Cas systems has transformed nucleic acid detection and manipulation. Cas13, a type VI CRISPR effector, targets RNA with high sensitivity through both cis (target RNA) and trans (collateral RNA) cleavage. This property enables the use of fluorescent reporters for sensitive diagnostics. However, Cas13's heightened sensitivity also leads to reduced specificity due to its susceptibility to...
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