Article

Accelerated RNA detection using tandem CRISPR nucleases

2021-03-24

Abstract excerpt

Direct, amplification-free detection of RNA has the potential to transform molecular diagnostics by enabling simple on-site analysis of human or environmental samples. CRISPR-Cas nucleases offer programmable RNA-guided recognition of RNA that triggers cleavage and release of a fluorescent reporter molecule 1,2 , but long reaction times hamper sensitivity and speed when applied to point-of-care testing. Here we sho...

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Literature Corpus work: fc3ddbc9-3864-56c0-a352-8a7e4cb69953
DOI: 10.1101/2021.03.19.21253328

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