Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection.

doi:10.1021/acs.analchem.5c07125

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Article

Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection.

Analytical chemistry - 3 Mar 2026

Xin Wenjuan, Tang Zijie, Wang Shumin, Song Zhi-Ling, Luo Xiliang

Abstract excerpt

The CRISPR/Cas12a system has revolutionized nucleic acid diagnostics, but its direct application for RNA detection remains constrained by the requisite reverse transcription step, insufficient sensitivity, and poor compatibility across diverse RNA targets such as microRNAs (miRNAs), long mRNAs, a...

Topics

DNA
CRISPR-Cas Systems
CRISPR-Associated Proteins
MicroRNAs
Polymorphism, Single Nucleotide
Endodeoxyribonucleases
Bacterial Proteins
RNA, Messenger

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