Article

Modular split-trigger cooperative activation enables a programmable Cas13-12 cascade

2026-06-27

Abstract excerpt

Achieving precise control of CRISPR/Cas trans -cleavage depends on understanding how nucleic acid activators engage Cas effectors, yet the fundamental principles of split-trigger activation of Cas12a remain unclear. Here, we uncover the mechanistic determinants that enable fragmented nucleic acids to collectively initiate Cas12a activity. We show that split triggers bearing external extensions fully support the R...

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Literature Corpus work: 066dea84-8154-5e90-81c8-bccd50720ddf
DOI: 10.64898/2026.06.26.734747

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