Back to search

Article

A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNA

2024-01-04

Abstract excerpt

The complete 40-nt CRISPR RNA (crRNA) of Cas12a can be artificially divided into two parts, including the 20-nt scaffold RNA with fixed sequences and the 20-nt spacer RNA with variable sequences, respectively. Herein, we found that Cas12a protein, scaffold RNA, and spacer RNA can be reassembled into an active ribonucleoprotein (RNP) which has a trans -cleavage activity comparable to that of wild-type Cas12a RNP....

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
0dfb7095-1903-5019-b396-0aaafdf30dda
DOI
10.1101/2024.01.04.574169
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNADOI 10.1101/2024.01.04.574169
A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNASemantic proximity
Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Dual-Functional CRISPR-Cas12aSemantic proximity
A general onepot-method for nucleic acid detection with CRISPR-Cas12aSemantic proximity
Modular split-trigger cooperative activation enables a programmable Cas13-12 cascadeSemantic proximity
A Novel Dual-Guide CRISPR-Cas13 Strategy Improves Specificity for Single-Nucleotide Variant DetectionSemantic proximity
Enhancement of CRISPR-Cas12a system through universal circular RNA designSemantic proximity
Development and evaluation of a CRISPR/Cas12a-based diagnostic test for rapid detection and genotyping of HR-HPV in clinical specimensSemantic proximity
Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection.Semantic proximity
Hybrid CRISPR/Cas protein for one-pot detection of DNA and RNASemantic proximity
Hybrid CRSIPR/Cas Protein for One-Pot Detection of DNA and RNASemantic proximity
Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Multifunctional CRISPR-Cas12aSemantic proximity
A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical SamplesSemantic proximity
Select a neighboring publication to make it the new centre.