Article

Harnessing Non-Canonical crRNAs to Improve Functionality of Cas12a Orthologs

2023-07-24

Abstract excerpt

There is a broad diversity among Cas12a endonucleases that possess nucleic acid detection and gene editing capabilities, but few are studied extensively. Here we present an exhaustive investigation of 23 Cas12a orthologs, with a focus on their cis- and trans-cleavage activities in combination with non-canonical crRNAs. Through biochemical assays, we observe that some non-canonical crRNA:Cas12a effector complexes o...

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Literature Corpus work: 20530b5d-9092-5971-8be7-13745d297c3e
DOI: 10.2139/ssrn.4515211

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