Article

From RNA to DNA: CRISPR/LbuCas13a Demonstrates Exceptional Single-Nucleotide Specificity

2024-02-19

Abstract excerpt

<title>Abstract</title> <p>Traditionally perceived as an RNA-specific nuclease, Cas13a has garnered extensive utilization in RNA detection. This paradigm is challenged by our discovery of LbuCas13a's ability to directly target DNA without the restrictions of (Protospacer Flanking Sequence) FPS and (Protospacer Adjacent Motif) PAM sequences, coupled with robust <italic>trans</italic>-cleavage activity, a breakthro...

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Literature Corpus work: e8d73eef-e0a0-5128-901a-e9903fed0674
DOI: 10.21203/rs.3.rs-3920513/v1

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