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Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics

2024-04-12

Abstract excerpt

<title>Abstract</title> <p>Cas12 and Cas13 are extensively utilized in molecular diagnostics for their <italic>trans</italic>-cleavage activities, yet their activation characteristics remain partially understood. Here, we conduct an in-depth investigation of Cas12a, Cas12f1, and Cas13a, uncovering the characteristics of their <italic>trans</italic>-DNase and <italic>trans</italic>-RNase activities with noncanonic...

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Literature Corpus work
e1af8d98-9413-555c-8abf-dec1c82e7f5a
DOI
10.21203/rs.3.rs-4164931/v1
Open publication

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Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnosticsDOI 10.21203/rs.3.rs-4164931/v1
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