Article

PAM-free diagnostics with diverse type V CRISPR-Cas systems

2024-10-23

Abstract excerpt

<title>Abstract</title> <p>Type V CRISPR-Cas effectors have revolutionized molecular diagnostics by facilitating the detection of nucleic acid biomarkers. However, their dependence on the presence of protospacer adjacent motif (PAM) sites on the target double-stranded DNA (dsDNA) greatly limits their flexibility as diagnostic tools. Here we present a novel method named PICNIC that solves the PAM problem for CRISP...

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Literature Corpus work: e3943c65-62e6-5527-8880-8fb6c89089fa
DOI: 10.21203/rs.3.rs-4355614/v1

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