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A Novel Dual-Guide CRISPR-Cas13 Strategy Improves Specificity for Single-Nucleotide Variant Detection

2025-09-27

Abstract excerpt

<h4>ABSTRACT</h4> The emergence of CRISPR-Cas systems has transformed nucleic acid detection and manipulation. Cas13, a type VI CRISPR effector, targets RNA with high sensitivity through both cis (target RNA) and trans (collateral RNA) cleavage. This property enables the use of fluorescent reporters for sensitive diagnostics. However, Cas13’s heightened sensitivity also leads to reduced specificity due to its sus...

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Literature Corpus work
b52ee959-6a2d-568b-9982-ed390612140f
DOI
10.1101/2025.09.24.25336526
Open publication

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A Novel Dual-Guide CRISPR-Cas13 Strategy Improves Specificity for Single-Nucleotide Variant DetectionDOI 10.1101/2025.09.24.25336526
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