Article

Expanding the DNA editing toolbox: novel lambda integrase variants targeting microalgal and human genome sequences

2023-09-26

Abstract excerpt

Recombinase enzymes are extremely efficient at integrating very large DNA fragments into target genomes. However, intrinsic sequence specificities curtail their use to DNA sequences with sufficient homology to endogenous target motifs. Extensive engineering is therefore required to broaden applicability and robustness. Here, we describe the directed evolution of novel lambda integrase variants capable of editing e...

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Literature Corpus work: e410bfc8-d826-5a2a-880a-b17f7e914962
DOI: 10.1101/2023.09.22.559039

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