Article

SyngenicDNA: stealth-based evasion of restriction-modification barriers during bacterial genetic engineering

2018-08-09

Abstract excerpt

Restriction-modification (RM) systems hinder the use of genetic approaches in the vast majority of bacteria. Here, we describe a systematic approach to adapt genetic tools for use in bacteria that are genetically intractable or poorly tractable owing to active RM defenses. In this process, we determine the genome and methylome of a bacterial strain and use this information to define the bacterium’s RM target motif...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: abc720a4-a22a-5b01-9374-0609f65e1dc0
DOI: 10.1101/387985

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

SyngenicDNA: stealth-based evasion of restriction-modification barriers during bacterial genetic engineeringDOI 10.1101/387985Read

Functional genomics reveals extensive diversity in <i>Staphylococcus epidermidis</i> restriction modification systems compared to <i>Staphylococcus aureus</i>Semantic proximity Read

Mining the Methylome Reveals Extensive Diversity in Staphylococcus epidermidis Restriction ModificationSemantic proximity Read

Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages.Semantic proximity Read

Genetic engineering of <i>Staphylococcus haemolyticus</i> : Overcoming Restriction-Modification Barriers and Targeting Virulence GenesSemantic proximity Read

One-for-all gene inactivation via PAM-independent base editing in bacteriaSemantic proximity Read

Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 ToolkitSemantic proximity Read

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species.Semantic proximity Read

Equip <i>Fusobacterium nucleatum</i> genetic tool kits with compatible shuttle vectors and engineered intermediatory <i>E. coli</i> strains for enhanced transformation efficiencySemantic proximity Read

Functional analysis of the first complete genome sequence of a multidrug resistant sequence type 2 Staphylococcus epidermidisSemantic proximity Read

MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.Semantic proximity Read

DNA methyltransferase enhanced <i>Fusobacterium nucleatum</i> geneticsSemantic proximity Read

Methylome-dependent transformation of <i>emm</i> 1 group A streptococciSemantic proximity Read