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Article

Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution.

Nature biotechnology - 1 Jun 2004

Ghadessy Farid J, Ramsay Nicola, Boudsocq François, Loakes David, Brown Anthony, Iwai Shigenori, Vaisman Alexandra, Woodgate Roger, Holliger Philipp

Abstract excerpt

DNA polymerases recognize their substrates with exceptionally high specificity, restricting the use of unnatural nucleotides and the applications they enable. We describe a strategy to expand the substrate range of polymerases. By selecting for the extension of distorting 3' mismatches, we obtained mutants of Taq DNA polymerase that not only promiscuously extended mismatches, but had acquired a generic ability to...

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PMID: 15156154
DOI: 10.1038/nbt974

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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