Article

Megabase-scale human genome rearrangement with programmable bridge recombinases

2025-05-14

Abstract excerpt

Bridge recombinases are a class of naturally occurring RNA-guided DNA recombinases. We previously demonstrated they can programmably insert, excise, and invert DNA in vitro and in bacteria. Here, we report the discovery and engineering of IS622, a simple two-component system capable of universal DNA rearrangements of the human genome. We define strategies for the optimal application of bridge systems, leveraging m...

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Literature Corpus work: 45ac483f-cc3e-5c85-9417-085dc0a4a8f7
DOI: 10.1101/2025.05.14.653916

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