Article
Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes.
Nucleic acids research - 1 Aug 2011
Li Ting, Huang Sheng, Zhao Xuefeng, Wright David A, Carpenter Susan, Spalding Martin H, Weeks Donald P, Yang Bing
Abstract excerpt
Recent studies indicate that the DNA recognition domain of transcription activator-like (TAL) effectors can be combined with the nuclease domain of FokI restriction enzyme to produce TAL effector nucleases (TALENs) that, in pairs, bind adjacent DNA target sites and produce double-strand breaks between the target sequences, stimulating non-homologous end-joining and homologous recombination. Here, we exploit the...
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