Article

Site-specific DNA insertion into the human genome with engineered recombinases

2024-11-03

Abstract excerpt

Technologies for precisely inserting large DNA sequences into the genome are critical for diverse research and therapeutic applications. Large serine recombinases (LSRs) can mediate direct, site-specific genomic integration of multi-kilobase DNA sequences without a pre-installed landing pad, but current approaches suffer from low insertion rates and high off-target activity. Here, we present a comprehensive engine...

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Literature Corpus work: 184e9331-0a72-54ad-93fb-ee7ae5fbb60e
DOI: 10.1101/2024.11.01.621560

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