Article

Off-target effects as confounders of Cas13d-based lncRNA screens

2025-11-29

Abstract excerpt

<h4>Summary</h4> Despite their recognized role in biology, a majority of the ∼100,000 lncRNA genes remain functionally uncharacterized. In a recent study ( Liang WW et al ., Transcriptome-scale RNA-targeting CRISPR screens reveal essential lncRNAs in human cells, Cell, 2024) , Liang et al. utilized the RNA nuclease Cas13d to perturb ∼6,200 lncRNAs in fitness screens across five cell lines - thereby identifying...

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Literature Corpus work: e39e576c-9fd4-5fda-b329-70aa315d33b7
DOI: 10.1101/2025.11.27.690751

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