Article
MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens.
Genome biology - 1 Jan 2014
Li Wei, Xu Han, Xiao Tengfei, Cong Le, Love Michael I, Zhang Feng, Irizarry Rafael A, Liu Jun S, Brown Myles, Liu X Shirley
Abstract excerpt
We propose the Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) method for prioritizing single-guide RNAs, genes and pathways in genome-scale CRISPR/Cas9 knockout screens. MAGeCK demonstrates better performance compared with existing methods, identifies both positively and negatively selected genes simultaneously, and reports robust results across different experimental conditions. Using public...
Topics
- Algorithms
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Cell Line, Tumor
- Computational Biology
- Gene Expression Regulation, Neoplastic
- Gene Knockout Techniques
- Genes, Essential
- Genetic Predisposition to Disease
- HL-60 Cells