Article

DANGER ANALYSIS: RISK-AVERSE ON/OFF-TARGET ASSESSMENT FOR CRISPR EDITING WITHOUT A REFERENCE GENOME

2023-03-12

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR-Cas9 system has successfully achieved site-specific gene editing in organisms ranging from humans to bacteria. The technology efficiently generates mutants, allowing for phenotypic analysis of the on-target gene. However, some conventional studies did not investigate whether deleterious off-target effects partially affect the phenotype. Herein, we present a novel phenotypic assessment...

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Literature Corpus work: 52fac0af-9eab-50a1-815e-e4228b5bda53
DOI: 10.1101/2023.03.11.531115

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