Article

Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH

2021-11-29

Abstract excerpt

<h4>ABSTRACT</h4> Extrachromosomal DNA (ecDNA) is a common mode of oncogene amplification but is challenging to analyze. Here, we present a method for targeted purification of megabase-sized ecDNA by combining in-vitro CRISPR-Cas9 treatment and pulsed field gel electrophoresis of agarose-entrapped genomic DNA (CRISPR-CATCH). We demonstrate strong enrichment of ecDNA molecules containing EGFR , FGFR2 and MYC f...

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Literature Corpus work: e1c8e547-fe50-5646-8ee0-fcd56c6bc23e
DOI: 10.1101/2021.11.28.470285

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