Article

A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures

2020-07-01

Abstract excerpt

<h4>ABSTRACT</h4> <h4>Background</h4> CRISPR-Cas9-based technologies have revolutionized experimental manipulation of mammalian genomes. None-the-less, limitations of the delivery and efficacy of these technologies restrict their application in primary cells. <h4>Aims</h4> To create an optimized protocol for penetrant, reproducible, and fast targeted insertiondeletion mutation (indel) formation in cell cultures...

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Literature Corpus work: 3368a45b-aa59-57be-9b35-70211bdec522
DOI: 10.1101/2020.07.01.183145

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