Article

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effect

2021-12-23

Abstract excerpt

CRISPR-Cas13 systems have recently been employed for targeted RNA degradation in various organisms. However, collateral degradation of bystander RNAs has imposed a major barrier for their in vivo applications. We designed a dual-fluorescent reporter system for detecting collateral effects and screening Cas13 variants in mammalian cells. Among over 200 engineered variants, several Cas13 variants (including Cas13d...

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Literature Corpus work: ddd7b0a9-6f67-5efb-b7ff-a61b878cfa78
DOI: 10.1101/2021.12.18.473271

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