Article

Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells

2023-01-19

Abstract excerpt

CRISPR-Cas13 exclusively targets RNA. In prokaryotic cells, Cas13 cleaves both target and non-target RNA indiscriminately upon activation by a specific target RNA, but in eukaryotic cells collateral cleavage activity has been limited. To investigate collateral cleavage by Cas13 in eukaryotic cells, we first compared various Cas13 orthologs and found that specifically LbuCas13a exhibits strong collateral RNA cleava...

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Literature Corpus work: ac9e6970-331e-5cd1-b10c-585bd1356138
DOI: 10.1101/2023.01.19.524716

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