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Article

Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryos

2026-04-08

Abstract excerpt

<h4>ABSTRACT</h4> Collateral RNA cleavage by CRISPR-Cas13 effectors presents a critical obstacle to their application in biological research and therapeutics, yet the molecular determinants of this activity remain poorly understood. Here, we systematically investigate the collateral activity of various Cas13 variants in human cells in vitro and zebrafish embryos in vivo. Among these nucleases, Rfx Cas13d display...

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Identifiers and source

Literature Corpus work
bf7f1479-4726-5a99-8a5a-fcbf1c98489d
DOI
10.64898/2026.04.07.716849
Open publication

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Target RNA abundance controls the collateral activity of RfxCas13d in human cells and zebrafish embryosDOI 10.64898/2026.04.07.716849
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